ANALYSIS OF EXISTING BIOREACTORS WITH IMMOBILIZED MICROORGANISMS

Array

Authors

Keywords:

bioreactor, immobilized, microorganisms, biotechnology

Abstract

This review article describes the current state of the industry of immobilized cells and the development of bioreactors with immobilized cells and carrier materials for immobilization. On the basis of literature analysis, a classification of constructions of bioreactors with immobilized cells is proposed, depending on the location of cellular aggregates: suspended particles, fixed particles and moving surfaces used with immobilized cells.

The modern development of biotechnological production requires new approaches to the implementation of biosynthesis processes. The performance of bioreactors in the production of various target products depends on the efficiency of the use of biological agents, which depends on the process conditions. One of the ways to improve the performance of fermentation is cell immobilization, which provides the possibility of continuous processing, cell stability, reducing the cost of recovery, recycling and further processing. Immobilization of cells protects them from landslide efforts that may occur during the operation of the bioreactor. At the same time, the industrial use of immobilized cells is still limited and their further application requires a detailed analysis of the specific features of the designs of bioreactors with immobilized cells and carrier materials for immobilization.

At present, there is a large variety of immobilization methods, but they can be conventionally divided into chemical, physical and mechanical. The most widespread mechanical method was the inclusion of cells in the composition of gels, membranes and fibers.

Consequently, for the wider introduction of methods and equipment using immobilized cells in biotechnological and pharmaceutical industries, further research is required on how to immobilize cells on a variety of carriers.

Author Biographies

V. Mel’nick, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”

Doctor of Technical Sciences, Professor

L. Rhuzinska, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”

Ph.D., Associate Professor

O. Vorobiova, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”

graduate student of Department

References

Higgins, I.J., Best, D.J., Jones, J. (1988) Biotechnology : Principles and Applications. Moscow, Russian: Myr, 480. [in Russian]

Sinitsyn, A.P., Rainina, E.I., Lozinsky, V.I., Spasov, S.D. (1994) Immobilized microbial cells. Moscow, Russian: Publishing House of Moscow State University, 288.

Onishi, J., Vakamura, O., Yamada, E., Kato, Yu., Murat, A. (2015). A bubble column reactor and a method of controlling a bubble column reactor C10G 2/00 (2006.01) № 21337 declared 25.09.2009; published 29.05.2015, № 3.

Schneider, R., Fritz, P. M., Mushelknautz, S., Bölt, H., Ali, T. (2011). The method of operation of the bubbling column reactor B01J 10/00 (2006.01) № 2410156 declared 06.09.2006; published 27.01.2011, № 3.

Nedovic, V, Willaert, R, Leskosek-Cukalovic, I, Obradovic, B, Bugarski, B. (2005) Beer production using immobilized cells. In: Nedovic, V, Willaert R, editors. Applications of cell immobilisation biotechnology. New York, NY: Springer, 259–273.

Park, D., Lee, D.S., Joung, J.Y., Park, J.M. (2005) Comparison of different bioreactor systems for indirect H2S removal using iron-oxidizing bacteria. Proc. Biochem, 40, 1461-1467.

Nemati, M., Webb, C. (1997) Does immobilization of Thiobacillus fer-rooxidans really decrease the effect of temperature on its activity? Biotechnol. Let., 19, 1, 39-43.

He, P, Greenway, G, Haswell, SJ. (2010) Development of enzyme immobilized monolith microreactors integrated with microfluidic electrochemical cell for the evaluation of enzyme kinetics. Microfluid Nanofluid;8:565–73. http://dx.doi:10.1007/s10404-009-0476-8

Liu, Y, Yang, D, Yu, T, Jiang, X. (2009) Incorporation of electrospun nanofibrous PVDF membranes into a microfluidic chip assembled by PDMS and scotch tape for immunoassays. Electrophoresis; 30:3269–75. http://dx.doi:10.1002/elps.200900128

Lab-on-a-Chip Fabrication and Application. (2016) Front Cover. Margarita Stoytcheva, Roumen Zlatev. BoD – Books on Demand, Jun 29, 2016 - Science - 208 pages. http://dx.doi.org/10.5772/61470

Zhang, H., Bruns, M.A., Logan, B.E. (2006) Biological hydrogen production by Clostridium acetobutylicum in an unsaturated flow reactor. Water Res. 40, 728–734.

Renewable and Sustainable Materials in Green Technology. (SpringerBriefs in Applied Sciences and Technology) Yhaya, M. F., Tajarudin, H. A., Ahmad, M. I. Springer; 1st ed. 2018 edition (March 16, 2018) 76 pages http://dx.doi.org/10.1007/978-3-319-75121-4

Byong, H. Lee (2015) Fundamentals of food biotechnology. – Second edition., 664.

Byong, H Lee at all (1995) Fundamentals of Food Biotechnology. - First Edition. 431.

Jain,, M. (2018) Anaerobic Membrane Bioreactor as Highly Efficient and Reliable Technology for Wastewater Treatment—A Review. Advances in Chemical Engineering and Science , 8, 82-100. https://doi.org/10.4236/aces.2018.82006

Liao, B.Q., Kraemer, J.T. and Bagley, D.M. (2006) Anaerobic Membrane Bioreactors: Applications and Research Directions. Critical Review in Environmental Science and Technology , 36, 489-530. https://doi.org/10.1080/10643380600678146

Osmanov, V.K. (2014) Engineering Enzymology [Product innovative policy]. N. Novgorod: Publishing house NizhGMaA. Retrieved from http://studopedia.su/19_90551_spisok-sokrashcheniy.html [in Russian]

Immobilized Cells: Principles and Applications. (1987) Front Cover. J. Tampion, M. D. Tampion. Cambridge University Press, Dec 10, 1987 - Medical – 264.

Published

2019-07-02

How to Cite

Mel’nick, V., Rhuzinska, L., & Vorobiova, O. (2019). ANALYSIS OF EXISTING BIOREACTORS WITH IMMOBILIZED MICROORGANISMS: Array. Municipal Economy of Cities, 3(149), 51–57. Retrieved from https://khg.kname.edu.ua/index.php/khg/article/view/5416